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plasmid replicon meaning in Chinese

质体复制子

Examples

  1. A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
    进一步序列分析表明该复制区至少有3个较大的orf ,分别编码501 , 333 , 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ,而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ,复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上, 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传,对受体菌株无明显不良影响。

Related Words

  1. dna replicon
  2. plasmid
  3. plasmids
  4. f plasmid
  5. multicopy plasmids
  6. plasmid analysis
  7. multicopy plasmid
  8. cryptic plasmid
  9. dna plasmid
  10. mobilizable plasmid
  11. plasmid recombinant
  12. plasmid replication
  13. plasmid rescue
  14. plasmid rescue technique
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